Sentence examples for sorted library from inspiring English sources
The sorted library IgGs further showed neutralization profiles which are similar to those of the serum IgGs, but they were overall less potent than the serum IgGs.
The sorted library IgGs and the serum IgGs bound weakly to the resurfaced Env gp120, RSC3, and did not bind to the CD4 binding site (CD4bs) knock-out mutant, ΔRSC3.
The resulting sorted library population was then amplified by growth in liquid cultures before expression of both TEVp and reporter substrate was induced.
Approximately 67% of the clones from the sorted library 3 actually contained the peptide, ENLYFQG, whereas the rest exhibited quite a diverse range of residues occupying the randomized positions.
Pools were screened using specific PCR markers previously developed from clones in the 3B sorted library.
However, the sorted libraries composing of HIV-1-specific neutralizing antibodies (nAbs) in the three recombinant libraries exhibited comparable binding and inhibitory activities, as well as antibody-dependent cell-mediated cytotoxicity (ADCC), to the serum IgGs.
Several individual clones from the sorted libraries, representing different substrate peptide sequences, were subjected to flow cytometry analysis in order to rank them on the basis of their whole-cell fluorescence intensity.
Although cancer, micro-dissected and sorted libraries in non-endothelial cell lines were thought to contaminate and invalidate the analysis, there exist many of these libraries in the public domain.
Thus, to maximise the chance of predicting a comprehensive set of endothelial genes, a final experiment (experiment 3) was performed using non-endothelial libraries that included cancer, micro-dissected and sorted libraries (178,653 ESTs and 733,461 SAGE tags, Additional file 11).
An aliquot of the non-sorted library emulsion was plated in the same way and used as a reference sample.
The positions of the seven newly selected BAC clones were confirmed on the basis of sequence identity with overlapping regions from the BACs derived from the 3B chromosome-sorted library and also genetic mapping of markers which were derived from these new clones.
