To analyze the expression of BCR-ABL1 in engrafted human cells, GFP+ and GFP− BM cells from BA mice were sorted into four populations: CD19+ B cells, CD3+ T cells, CD33+/CD14+/CD15+ myeloid cells and one population negative for all of these markers called 'other'other
Lakadamyali, M., Rust, M. J. & Zhuang, X. Ligands for clathrin-mediated endocytosis are differentially sorted into distinct populations of early endosomes.
In HT29 the CD133 protein was knocked down and cell numbers monitored for 5 days (Figure 4a) whilst SW480 was sorted into CD133+/CD133− populations and cell numbers were monitored over 9 days (Figure 4b).
Cells were sorted into four populations; those negative for both GCTM2 and TG30, and cells that exhibited low level, mid-level or high level expression for both TG30 and GCTM2.
Images were acquired using Zeiss Axioimager.Z1 and Axiovert 200 microscopes, both with epifluoresence capability and fitted with an Axiocam MRm camera and Axiovision 4.6 software (Carl Zeiss, Inc .. Cultures of unfixed ES cells were sorted into four populations for parallel analysis of gene expression and development in cell culture.
Parent H357 cells were therefore labelled with Hoechst 33342 dye and sorted into two populations termed SP(1) and G2(1) based on their relative blue/red fluorescence intensity.
(A ) Schematic of animals from a chemotaxis assay washed and sorted into two populations based on successful or failed navigation up the BZ odor gradient, for lifespan analysis.
Cells were sorted into separate populations based on CD24 expression and assessed for luminal epithelial and myoepithelial/basal markers by direct fluorescent microscopy and real time PCR.
Cells sorted into distinct populations based on the expression of epithelial and progenitor cell markers (CD34 and EpCAM) showed no difference in self-renewal in 2D culture, either as whole populations or as single cells.
(A ) Schematic of animals from a chemotaxis assay washed and sorted into two populations, based on success or failure in navigating up the BZ odor gradient, for calcium imaging.
