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CD150hi-HSC, CD150lo-HSC, and CD150neg-MPP were sorted as above into 500 µl of TRIzol reagent (Life Technologies, Grand Island, NY, United States).
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Populations were sorted as described above and treated for 24 h with 0.5 μmol/L Doxo.
Cell subsets were sorted as described above.
BM Lin- progenitors and HSCs used for qRT-PCR were sorted as described above.
Transfected (GFP+) and non-transfected (GFP−) cells were sorted as described above.
We can assume that the μ-vectors of each network are sorted as explained above.
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