Our other luciferase reporter vector (7TFC) allows sorting of infected cells when drug selection is not possible, and the 7TP virus follows a novel strategy to directly isolate cells with high Wnt signaling activity.
Flow cytometry allows the analytical, and physical (by fluorescently activated cell sorting), separation of infected macrophages and therefore this population can be separately assessed for immune regulatory changes.
To study antigen-presenting capacity of NK cell subsets and compare it to cDCs, all cell subsets were sorted from lungs of infected mice and co-cultured ex vivo with antigen specific T cells.
Subsets of conventional NK and IKDCs were sorted from the lungs of infected mice to study their APC potential, in direct comparison with conventional DCs.
With the goal of screening approximately 10 gene trap events, 10 cells exposed to virus were harvested for sorting, assuming that approximately 10% of infected cells will have true gene traps.
Low virus titers may have also been caused by contamination of infected mosquito fragments during sorting and testing procedures.
Moreover, expression of mCherry allows for selection of infected cells by fluorescence activated cell sorting (FACS).
We thank Mr. Ernest M. Meyer at the UPCI flow core facility for sorting of HIV-1 infected DC.
The recovery of homogeneous populations of infected and uninfected neutrophils from the skin by sorting the eGFPhiRFP+ and eGFPhiRFP− cells, and the accelerated apoptosis of the L. major-infected neutrophils, have been previously described.
Aside from infected Microsoft Word documents, what sort of things could it be scanning for?
