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In one sort experiment, BMP inhibitor Noggin (500 ng/mL, R&D Systems) and TGF-beta inhibitor SB431542 (10 μmol/L, Sigma-Aldrich) were included during EB differentiation stages prior to their attachment onto poly-L-ornithine/laminin coated dishes.
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AT- and SN-cells are shown regarding CD34 and CD61 expression using gates according to sort experiments.
(c) FACS analysis of CD34+CD61+ and CD34+CD61- sorted cells after 5 days in ECM culture to CD61 expression using gates according to sort experiments.
GFP-positive cells were sorted using three different gates as shown in the Results Section and sorted cells (50-300 cells per sorting experiment) were collected in ~300 μl complete culture medium at room temperature.
In order to assess the impact of the consumers' knowledge and experience onto their perception of wine glasses, a free sorting experiment was undergone.
The particle sorting experiment showed good separation results when the designed channel and magnetic tweezers were used.
A preliminary sorting experiment highlighted 9 main categories of sounds connected with these different mechanisms and properties.
In another sorting experiment, we have sorted CD3+ T cell population to compare with all the 4 subsets of B cells as well as with total PBMC.
sThe cell sorting experiment of DIO-SGC7901 anDID-hucMSCsCs was also tested (Additional file 1).
A lymphoblastic cell line was cultured to achieve the high number of cells needed for a flow sorting experiment.
FACS sorting experiment was previously described (Lombardo et al).. Cells were pre-treated and seeded in 6-well plates for 72 hrs.
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