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Subsequent studies using more sophisticated sample preparation technique have demonstrated a more comprehensive map of the BALF proteins [79],[80],[83] resulting in creation of a database of BALF proteins [81],[81].
Other methods, such as 3D tissue printing (Villar et al. 2013) and photo-sensitive hydrogel (Hahn et al. 2006; Kloxin et al. 2009; DeForest and Anseth 2011; Applegate et al. 2015) are capable of 3D cell-ECM patterning at the price of expensive equipment, non-native ECM composition, or sophisticated sample preparation (Yanagawa et al. 2016).
In response to this five-year study, I have conducted my own five-minute study, Celeste Fine's Study of Reading, in which I similarly interviewed a much more sophisticated sample of seven people from my Tuesday night pool team and discovered that this New Literacy has also significantly increased how much we read every day.
HPLC requires sophisticated sample preparation that is expensive and time consuming and requires trained chemists for analysis and interpretation of results.
However, the required sophisticated sample preparation steps and associated costs make that approach currently more attractive for patient samples than for cost-sensitive high-throughput stem cell donor registry typing.
Purification of the analytes is often achieved by using sophisticated sample cleanup approaches with subsequent separation by liquid chromatography and detection using triple-quadrupole analyzers coupled via an electrospray ionization (ESI) interface.
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Proponents say that with the increasingly sophisticated sampling techniques of digital technology, there is virtually no difference in sound between a true organ pipe and its digital impersonator.
Compared with some groundwater dating techniques, CFCs can be determined rapidly without sophisticated sampling and analytical instrument.
Our approach uses people as components of a Markov chain Monte Carlo (MCMC) algorithm, a sophisticated sampling method originally developed in statistical physics.
As such, PHS aims at benefitting statisticians whom, working on a wide spectrum of applications, are more focused on defining and refining models than constructing sophisticated sampling strategies.
Since the early 1990s, a new team of faunal experts led by Nerissa Russell of Cornell University in Ithaca, New York, and Louise Martin of the Institute of Archaeology in London has been using more sophisticated sampling and analytical methods, relying not just on size but also on the sex and age patterns of the cattle.
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