And it concludes that researchers should soon adopt "tissue culture methods... as the routine method unless there is a clear reason they cannot be used".
Moreover, because miRNAs are short and single-stranded, they are unstable and likely to be degraded soon after tissues or cells are lysed, thereby compounding the difficulties in isolating and detecting these RNAs.
It is expected that problems like this will become important as soon as tissue-specific networks of human metabolism and cancer-specific networks will become available in the near future.
Ordinarily, scientists can collect tissue soon after a victim's death and produce a DNA profile within hours.
Our studies indicate that immunologically relevant antigen can be detected in both local and systemic lymphoid tissue soon after feeding antigen in tolerogenic or immunogenic forms.
If blood flow is restored to the affected tissue soon enough, cerebral ischemic damage may be reversible; however, if blood flow is not restored, the tissue dies resulting in irreversible damage.
This steep rise in collagen type VI expression in native tissue soon levels off at later stages of chondrogenesis, coinciding with an upregulation in collagen type II [10].
C3 is expressed in the local micro environment of stressed tissue soon after IRI which leads to the deposition of C3d on the plasma membrane of affected cells and can remain for several days.
Ideally a biomarker which can delineate the area at risk and the border zone around necrotic tissue soon after IRI, which also remains for several days, would be a powerful tool in risk stratification and guiding intervention.
Xenograft tumours were removed from the surrounding tissue soon after mice were killed by CO2 overdose.
