RT PCR showed some interindividual variation of expression, but the number of markers identified allowed us to discriminate between SEM and N-SEM samples.
Moreover, we observed a high level of agreement when comparing the variation of expression levels within the dataset (Spearman R = 0.902, p-value < 1 × 10−20) (Supplementary Fig. 1).
These images have been captured in regulated illumination, and the variation of expression is moderated (Figure 6). Figure 6 Samples from FACES94.
These data indicated that the variation of expression of ribosomal protein genes caused by replication within one tissue was much smaller than the variation between tissues.
mRNA gene expression data from 25 cell lines (see Table S1) were analyzed for variation of expression levels across the cell lines.
Our other finding of a temporal variation of expression of CB1 in the Fallopian tube reflects that of many genes in the endometrium suggesting a role for progesterone in the human endocannabinoid system [24], [25].
These genes present significant variation of expression amongst tissues.
Transcripts showing minimal variation of expression across the set of arrays were excluded from the analysis.
This suggests that large inter-individual variation of expression levels can obscure group-specific differences.
We detected variation of expression for very few genes (adjPv<0.01, Table 4).
