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This was necessary because results from some samples fell below the limits of detection or quantification (LOD or LOQ) of the analytical method or, in the case of the VMD data, values below a certain threshold were not reported (see above).

Amplified fragments were purified and sequenced, and mutations were detected in some samples (see below).

In those cases we substituted the value of the detection limit for the data point; so, for cases where some samples were below detection limit, our reported averaged values are an upper bound to the true average value.

Although the A260/A230 ratio of some samples was below 2.0 indicating the presence of guanidine thiocyanate, it has been shown that guanidine thiocyanate has no measurable effect on downstream applications such as RT-qPCR until concentrations of more than 100 mM [ 26] Moreover, RNA quality was assessed by 1% agarose gel electrophoresis.

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Below, some samples for the draft survey... What percentage of your new year's resolutions have been maintained until the end of the year?

Because some samples had values below the limits of detection, we modeled the missing data in a multivariate normal hierarchical Bayesian simulation model with conjugate noninformative priors using WinBUGS (Windows-based Bayesian inference Using Gibbs Sampling) (Spiegelhalter et al. 2003 ).

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The significance of the italicized phrase here will become clear when we take up some sample games below.

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