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The exception was that for some replicates of B. californica, due to low levels of natural recruitment, colonies were collected from the docks by hand and were connected to tiles with wire.
Still, some replicates of both AnimB and SireDam showed a tendency towards overestimated heritability.
Relative copy numbers among target groups were evaluated, and some replicates of apparent discrepancy were excluded in order to decrease standard error.
This may also be a reason why methanogenesis was progressively reduced in some replicates of these inocula, since methanogens grow poorly at pH less than 6.0 and would possibly have begun to be washed out of the semi-continuous fermenter.
While large regions with a single genotype were consistent with some minor variation in imputed breakpoint position, the genotype of smaller regions varied between some replicates of samples covered in both the HincII and RsaI datasets (Additional file 8: Figure S7).
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The ACB-PCR assay was able to detect as few as 1% mutant alleles, but often presented some reproducibility problems, i.e. some sample replicates of the ACB-PCR assay showed ambiguous or even contrasting results between replicates.
These same two products also were observed after PCR amplification of cDNA from some biological replicates of S. cernua (data not shown).
The dilution at which amplification of some replicates dropped out was then chosen for further analysis.
Replicates of some representative experiments, which were run both in lab and pilot-scale reactors, indicated excellent reproducibility of the polymerization process.
CEPH genomic DNA, negative controls and replicates of some samples were included on each plate to assure consistency of the genotype calls.
PROC GENMOD and GLM were used for the field data because we could not fit genotype terms since all replicates of some genotypes died in the saline gardens.
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