Indeed, the exploratory analysis using the recombination detection programme (RDP2) had at least one method detect some recombinant sequences (TAN/60/99 and K66/80).
Due to the defect in glycosylation, functions of some recombinant glycoproteins are impaired (Xu and Ng 2015).
In some cases, early induction increases soluble protein production of some recombinant proteins by limiting the culture growth rate (Delisa et al. 2001).
Some recombinant proteins can be secreted by Tat pathway, but not by Sec system (Barrett et al. 2003; Dong et al. 2013).
Three major recombinant junctions (RJ) are present in the genome of the recombinant PVYNTN at sites HC/Pro-P3, 6K2 NIa, and the C-terminal region of CP gene and one RJ at HC/Pro-P3 site in some recombinant PVYN isolates (termed PVYN O).
However, some recombinant proteins such as Bm95, E6/E7, OprF-OprI and EspA-Stx2A1 with low purity of 80 90% also display the effective immunogenicity (García-García et al. 2000; De Bruijn et al. 1998; von Specht et al. 1995; Cheng et al. 2009).
However, we noticed that some recombinant ceQORH protein was lost, since already eluted from the matrix using filtration and washing buffers (Figure 4B, lanes F and W).
Recently, some recombinant TMV, such as TMVFN20 that contains an epitope FN20 from the FMDV VP1, were found to induce local necrotic lesions (LNL) on the inoculated leaves of a susceptible tobacco, Nicotiana tabacum Samsun nn.
We also showed that recombinant segments were randomly distributed in the M66-2 aN16961961 lineages, and those attributed to the O/MN divergence are probably also randomly distributed, but there is some distortion probably because with 32% of the genome affected, some recombinant segments overlap (see Supporting Methods in Text S1).
Additionally, limiting analysis to recombinant major allergens potentially restricts diagnosis to just over half of the affected population [39] and due to the lack of post-translational modifications, some recombinant allergens may not display the same immunological reactivity as their native counterparts [40].
