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Because some probe sets identify the same gene, the number of genes that is down-regulated is 103 and up-regulated 51, respectively.
Some probe sets correspond to homologs of S. pombe and S. cerevisiae, and are expected to be expressed in both.
For the scatter plots obtained between different treatments and cultivars, some probe sets fell above or below diagonal lines, indicating their differential hybridization intensity which corresponds to variation in gene expression (illustrated by colored boxes in Figure S2).
This might have led to an erroneous estimation of the specific variability of some probe sets.
For some probe sets, we might find probes indicating differential expression in opposing directions.
We validated the microarray expression values of some probe sets of particular interest for our research by qPCR.
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Figure 2 shows some probe set examples with a diurnal pattern identified.
A transcriptogram for these data is produced by taking the average of the expression levels over a window of radius r on the ordered list, that is, 5 where θ j = 1 if the gene at position j is a target for some probe set of the microarray platform used to generate the transcriptome, and θ j = 0 otherwise.
Some probes sets could not be uniquely annotated and were therefore not used in the functional enrichment analyses.
The exclusion of some probe-sets was undertaken on the basis that non-changing genes provide little information in a gene co-expression setting.
We also did not attempt to determine whether polymorphisms led to changes in exon boundaries, and so some probe-sets annotated as being within coding regions may actually be intronic or intergenic, and vice-versa.
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