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Some of the validated differential splicing events are shown in Fig. 6.
Subgroup analysis revealed that some of the validated associations differed by ER or PR status.
Semi-quantitative RT-PCR results of some of the validated DEGs are given in Supporting Information Fig.
The differences between the "Matching" and "Recovered" columns of Additional file 1: Table S14 are due to the alternative splicing displayed by some of the validated fusions [ 36, 37]; Barnacle treats fusion isoforms as distinct predictions, so makes multiple predictions for some of the validated fusion gene pairs.
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A finite element model is developed to predict and optimize the performance of the proposed silencer with some of the results validated experimentally.
In order to understand the mechanism of regulation of some of the previously validated genes identified in the microarray experiments, specific inhibitors for the three branches of the MAP kinase pathway namely the p38, the ERK and the JNK pathways were used prior to TGF-β treatment of the cells and expression of these genes was studied by real time qRT-PCR estimation.
Responsiveness has been examined in some of the studies validating EORTC QLQ-CR38 [ 1, 15].
In addition, some of them were validated by the stem-loop RT-PCR method (Figure 6).
Most researches adopt the eigenvalue analysis method to study the small signal stability of DFIG, and some of the conclusions were validated through time domain simulation.
For some of the genes, we validated the microarray data using this pipeline by FISH.
Some of the predictions were validated by Real-time RT-PCR and or RNase protection assay.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com