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The other oscillator is a small network with a self-modulated excitatory input to an excitatory neurone whose episodic firings synchronise some neurones of the first network chains.
In some neurones, there were individual rounded membrane-bound melanosomes (Fig. 1i).
In some neurones, cannabinoids have been shown to inhibit presynaptic voltage-dependent Ca2+ channels (e.g. see Kushmerick et al., 2004).
Kir7.1 is expressed in visceral tissues and some neurones within the CNS (Krapivinsky et al, 1998; Nakamura et al, 1999; Ookata et al, 2000; Shimura et al, 2001), although little is known about its functional role.
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NK1R are also expressed by some serotonergic neurones in the raphé nuclei (Lacoste et al. 2006), which is thought to gate motor activity (Jacobs & Azmitia 1992).
Although the major FLS cell type was morphologically different from DRG neurones, some cells could not be identified.
In addition, in some experiments, DRG neurones were cultured in medium containing only the supernatant of FLS cells.
ABHD6 was expressed also in many principal glutamatergic neurones, some GABAergic inteneurones, as well as astrocytes but not in resident microglia (Marrs et al. 2010).
It has, for example, been successfully used to transfect neurones (some of which are notoriously difficult to transfect), cells deep in tissues (DNA can be carried considerable distances through other cells such as layers of skin), and bacteria [ 4- 7].
Once covered with snail Ringer (flow rate 1.0 1.2 ml min−1, bath volume 0.1 ml) the inner connective tissue was torn with a fine tungsten hook to expose some of the neurones.
This assumption does not take into account compensatory mechanisms that serve to regulate [Ca2+]i homeostasis, thus maintaining some degree of neuronal function in senescent neurones or during acute insults such as stroke (Murchinson & Griffith, 1998; Verkhratsky & Toescu, 1998; Lee et al., 1999; Griffith et al., 2000; Pottorf et al., 2000a, 2002).
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