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We measured somatic size from fluorescence images acquired during recording, filling neurons with Alexa 488 via the recording pipette (figure 4A,B).
Neurons infected with sponge had no overt differences in somatic size or dendritic morphology compared to cells infected with the pRubi control retrovirus (Fig. 5b).
Somatic size was similar for ChAT+ and ChAT− neurons (ChAT+ neurons were 35.3×15.8 µm; ChAT− neurons were 29.7×13.5 µm; table 1).
MSNs were identified by somatic size and typical basic membrane properties (input resistance, membrane capacitance and time constant).
Instead, reduced somatic size and loss or thinning of dendrites and spines are the main contributors (Klapstein et al., 2001).
Cells also were identified by somatic size, basic membrane properties [Rin (input resistance), membrane capacitance and time constant], and by addition of biocytin (0.15%) to the internal solution.
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Thus, the more ventromedial cells had smaller somatic sizes and fewer dendrites.
These losses were accompanied by altered somatic cell size that affected the remaining neurons of all neuronal subtypes studied here.
Another influence on somatic replicon size is the ratio of Cdc45 to preRCs.
We predicted that the biological characteristics of mammalian somatic replicon size, usage, and origin efficiency might be derived at the molecular level from unique characteristics of preRC distribution or subunit availability that differs from embryonic extracts.
These results provide a molecular explanation for several biological characteristics of DNA replication in mammalian cells, including why somatic replicon sizes are large, origin efficiency is low, and replicon usage is staggered.
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