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Calibration curves were established and shown in Figs. 2 and 3 (A solvent standard, B standard addition) for cathinone and mephedrone, respectively.
Cathinone and mephedrone were diluted either in methanol as "solvent standard" or in plasma with known concentrations (0, 10, 33, and 100 ng/ml) of drugs as "standard addition" to at least five different calibration points (0 250 ng/ml).
Concentrations of cathinone and mephedrone were then calculated by subtracting the value of X-axis intercept of solvent standard calibration from the value of X-axis intercept of standard addition calibration (Tables 1 and 2).
$$\end{document} R A % = slope spiked samples slope neat solvent standard × 100.
$$\end{document} S S E % = slope matrix‐ matched standard slope neat solvent standard × 100.
The accuracy of response of DCP in blood or water was similar and was 94 102% of the solvent standard.
Similar(47)
Plasma samples containing cathinone and mephedrone (10, 100, and 500 ng/ml) were extracted using Strat-X™ Drug B cartridge and analyzed by LC-MS/MS together with solvent standards.
There are three commonly used techniques used to quantify the unknown samples: (1) external calibration with solvent standards, (2) external calibration with matrix matched standards, and (3) standard addition.
Solvent standards were also analyzed.
Avstd = average ion ratio based on solvent standards with ±30%% relative ion ratio tolerances (dashed lines ).
This was verified for a subset of 130 pesticides in solvent standards.
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