Solvent reference spectra were digitally subtracted from protein CD spectra.
All spectra were recorded at room temperature except of samples in DMSO- d6 and D2O (standard 35 °C) and where indicated otherwise and were referenced internally to solvent reference frequencies.
Proton chemical shifts are reported in ppm relative to internal tetramethylsilane (TMS, 0.0 ppm) or with the solvent reference relative to TMS employed as the internal standard ([D6]DMSO: 2.50 ppm; D2O: 4.79 ppm).
Solvents, reference standards, and other chemicals used in analysis of serum samples, as well as the instrumental support of the work, have been reported previously (Sjödin et al. 1999; Thuresson et al. 2005).
The assay mixture (1 mL) contained: 0.1 mM linoleic acid, the sample (or the same quantity of solvent as reference) and 50 mM sodium phosphate, pH 6.8.
The UV visible absorption spectra of the mineral sample were measured using Shimadzu UV spectrophotometer (M160 PC) at wavelength number range from 200 to 900 nm at room temperature using dimethylsulfoxide (DMSO) as a solvent and reference.
UV vis spectra of the prepared polymer sample were measured using Shimadzu UV spectrophotometer (M160 PC) at room temperature in the range 200 900 nm using dimethyl-formamide as a solvent and reference.
Proton nuclear magnetic resonance (H NMR) spectra were taken in D2O using a Varian 400 MHz or 500 MHz spectrophotometer, using the solvent as reference signal.
After 48 hr, cells were treated with 17β-estradiol (E2 -reduced medium alone (media control), E2-reduced media plus 0.1% DMSO (solvent control), reference agonist (10−10 M E2), or various dilutions of air sample extracts.
C NMR spectra are reported in ppm from tetramethylsilane by using the central peak of the solvent as reference (CD2Cl2: δ=54.0 ppm, [D1]TFA: δ=116.6 ppm), multiplicity with respect to proton (deduced from APT experiments, s=quaternary C, d=CH, t=CH2, q=CH3).
After 24 hr, cells were treated with growth medium [α-minimal essential medium (αMEM) plus 10% fetal bovine serum (FBS); media control], growth medium plus 0.1% dimethyl sulfoxide (DMSO; solvent control), reference agonist [10−7 M β-naphthoflavone (β-NF ], or various dilutions of the air sample extracts.
