The Shimadzu (Kyoto, Japan) LC-MS/MS equipment consisted of an LC-10ADVP binary solvent delivery pumps, SLC-10AVP system controller, SIL-20A Prominence auto sampler and CTO-10ASVP column oven set at 25 °C.
The solvent delivery systems are able to drive the mobile phase without flow splitters and promote gradient elution using either dual piston reciprocating or syringe-type pumps.
The classical LC system was first adapted to microbore-chromatography (internal diameter 1 mm) without apparatus miniaturization of the solvent delivery system and the detection cell.
GPC was equipped with a Waters 6000 solvent delivery system, RI detector, and a Rheodyne injector.
The Solvent Delivery Module fed samples of the remnant suspension continuously to the measurement unit, which consisted of a UV/vis spectrophotometer at a wavelength of 225 nm.
The chromatographic system consisted of a Waters e2695 Solvent Delivery Pump (Waters, USA) and a PDA-2998 UV-detector (Waters, USA).
Liquid chromatography was performed with an Agilent 1200 system (Agilent Corp., MA, USA), equipped with a binary solvent delivery system and an autosampler.
The instrument used was Waters ACQUITY UPLC™ (Waters Corp., MA, USA) with attached binary solvent delivery system and tuneable MS detector.
A single line FIA-SPEn manifold, illustrated in Figure 2, consisted of two solvent delivery pumps (Agilent 1100 Series Iso pump G1310A).
Glycerol (160 mM) solution was continuously supplied to the mixture at a flow rate of 1 μl min−1 (0.04 μmol glycerol ml−1 min−1) using a Shimadzu LC-20 AD solvent delivery unit.
The UPLC-ESI-MS system was performed on a Waters Acquity UPLC system (Waters Corp., Milford, MA, USA), equipped with a binary solvent delivery system, a conditioned autosampler at 4°C and a photo-diode array detector (PDA) system.
