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Our results showed that S228 stabilized two interacting loops to shield the CTD hydrophobic core from solvent access.
It will be interesting to investigate whether the other globular domains also possess similar structures to shield the hydrophobic core from solvent access.
Prior cell drying or cell disruption is necessary for current industrial-scale extractions because unruptured cells do not permit solvent access to the internally stored lipids.
We propose that these two interacting loops play an important role in the folding and structural stability of β/γ-crystallin domains by protecting the hydrophobic core from solvent access.
A water channel inside these fluorescent protein is suggested to open up solvent access to methylene/imidazolinone moieties of chromophores, allowing for enhanced generation and release of ROS, which in turn results in photobleaching and phototoxicity [44, 85, 110].
In such a model, binding of ToMOD to mutant Q228A would not protect the diiron center from solvent access.
Similar(37)
This condition is best fulfilled when the width of the binding region is large, and channel-solvent access dynamics is fast, see Eq. (20).
In the future, it is planned to keep developing PTM-SD by adding filters related to structural data, as resolution, R-Factor, B-Factor, solvent accessibility, by giving direct access to the coordinate data of PTM atoms, and by providing other options for the clustering tool.
A test compound featuring azido and cyano end groups linked by an alkyl spacer, az- CH2 11-CN (az- CH2 11-CNdesigned az- CH2 11-CN mobility of synthetic lipid membraz11CNteriors and mobility of solvents accessed via 2DIR spectral diffusion measurements.
The t1/2 values of His45 in DHFR-MTX, DHFR-MTX-NADPH and DHFR-folate-NADP+ were lower than apo-DHFR, suggesting that His45 has greater solvent accessibility (ability of bulk solvent to access the particular histidine imidazole ring) in the binary and tertiary complexes than in apo-DHFR.
The arrows indicate the point of access to the domain interface with potentially increased solvent accessibility.
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