Thereafter, 177Lu was added to the solution under agitation to obtain a homogeneous activity distribution.
To make chitosan gelatin scaffold, 4% (w/v) gelatin solution was added into the solution under agitation for 12 h at 37 °C.
2.2 mL of triethylamine was mixed with 7.8 mL of DMF and was slowly added drop by drop to the solution under agitation.
The estimated amounts of ibuprofen loaded in the samples after 24 hours of impregnation under hexane solution (under agitation at 25 °C) were 678, 828, and 954 mgibu/mgsample, respectively (Table 1).
The reaction was initiated by adding 100 μL of xanthine oxidase solution under agitation.
The cross-linked gelatin hydrogels were immersed in a 50 mM glycine aqueous solution under agitation for 1 h to block the residual aldehyde groups of glutaraldehyde, followed by two washes in double-distilled water for 1 h.
Under appropriate modification conditions involving stepwise-treatments in the Ca-and-P supersaturated solution under gentle agitation, a thin, poorly crystallized CaP layer was deposited.
The propofol-loaded mixed micelles were prepared by mixing propofol and the prepared drug-free micelle solution under magnetic agitation at room temperature.
Gels were stained in 0.5 μg/mL ethidium bromide solution under gentle agitation for 30 min.
The tissue was then digested by using 300 unit/ml collagenase type I solution under constant agitation for 90 min at 37°C.
