Finally, after cooling at room temperature, water was added to the solution to complete the volume of the volumetric flask.
The culture dish was rinsed with additional 100 µl of Triton solution to complete the harvesting of cells.
Therefore, we decomposed the binding free energy calculation into several steps in which the E2 is annihilated (i.e., decoupled) from its bound state in the receptor complex and then made to reappear in solution to complete the thermodynamic cycle.
Pt wire counter electrode and Ag/AgCl reference electrode were immersed into the electrolyte solution to complete the three-electrode cell.
The rat antihuman IL-2 antibody (eBioscience Inc; diluted 1 : 1000) was used as secondary antibody (wells A H), followed by the goat antirat IgG-HRP (eBioscience Inc.; diluted 1 : 1000), the POD substrate (Roche Diagnostic, Rotkreuz, Switzerland), and the H2SO4 1 solution to complete the reaction.
However, a booth in the waiting room may provide an ideal solution to completing measures in the waiting room area whilst still maintaining privacy.
The explicit finite difference [24] technique with stability and convergence analysis has been employed as a solution technique to complete the formulation of the unsteady model.
The manager of learning prepared and present unexpected tasks or challenges to the leader and the group which they must organize themselves to find a solution or to complete a task.
After 1 hour of incubation at 56°C the collection device was centrifuged and the resulting filtrate solution used to complete the extraction process, taking up the procedure at the protein precipitation step and completing as described previously.
To address solution lengths, we considered the lengths of "solutions:" sequences of interventions required to complete the solution (or non-solution) of an individual virtual patient in the mechanical ventilation data.
