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All single or mixed plasmid solutions subsequently used as PCR templates in the present study were prepared from these reference stocks.
The brain was postfixed overnight in 4% paraformaldehyde in PBS, pH 7.2, cryoprotected with 15%and25%5% sucrose solutions (subsequently overnight), frozen in 2-methylbutane (30 seconds at −42°C) and transferred to dry ice.
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One patient who received the medication, called cardioplegic solution, subsequently developed a fungal infection, the agency said.
If the reduction reaction is conducted in a separate solution, the as-prepared Ag nanostructures will be separated and added into the polymer precursor solution subsequently.
For RNA extraction of minute tissue samples, tissues were once fixed with ethanol-acetate (3∶1) solution, and the solution subsequently substituted for PBS (Phosphate Buffered Saline) with 10% sucrose [16].
This peroxide solution subsequently releases oxygen, thereby enabling a mechanical cleaning by the oxygen bubbles in addition to the chemical cleaning [ 52].
diluted 1 : 200 in blocking solution, subsequently identified by an HRP-conjugated secondary antibody (Bio-Rad Laboratories) and finally detected by a chemiluminescent substrate of HRP (Pierce Biotechnology , Inc.
One of them, an alternative solution subsequently known as Variant C, entailed a unilateral diversion of the Danube by Czechoslovakia on its territory some 10 kilometres upstream of Dunakiliti.
Then this solution was diluted into 6%, 33 %, 1 % by adding the whole solution up to 150 mL, 300 mL, and 900 mL with added buffered formalin solution subsequently.
The structured emulsion was fabricated by mixing lipid droplets, alginate solution, and subsequently chitosan solution.
In this process, the drug is dissolved in a solvent and this solution is subsequently added to a non-solvent solution.
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