Sentence examples for solutions is applied for from inspiring English sources
A hybrid evolutionary computation approach consisting of genetic algorithm for routing allocation with minimum degree first for wavelength assignment (GA-MDF) and the fast non-dominated sorting genetic algorithm (NSGA-II) to search for non-dominated solutions is applied for solving the multi-objective RWA network design problem.
Drug solutions were applied for 500 ms or 1 s every 3 min. The normalizing concentration of nicotine (75 μM) was applied several times to each cell during the course of an experiment to check for desensitization and/or rundown.
To evaluate α-T toxicity (α-T+ H2O2−), the α-T solutions were applied on cultured cells for 60 minutes; to evaluate α-T protective effect against H2O2 aggression, the solutions were applied for 60 minutes and then aspirated, followed by H2O2 application for 30 minutes (α-T+ H2O2+).
For the standard control, the same amount of water instead of test solution was applied (for both test substances), while for the solvent control an equal amount of acetone-treated quartz sand was applied (only for florfenicol).
The wells were washed thoroughly and then the secondary antibody conjugate solution was applied for 30 min with gentle agitation.
The primary antibody diluted in blocking solution was applied for one hour, except E-cadherin which was stained overnight at 4°C.
The ABC solution was applied for 1 hour at room temperature, after which slides were washed and the stain developed with diaminobenzidine (DAB; Sigma Aldrich)/0.01% H2O2/PBS.
After transfer and blocking with 5% skimmed milk in TBS, the primary antibody diluted in blocking solution was applied for 2 hours at 37°C.
After three washes in PBS (5 min each), the secondary antibody, diluted in blocking solution, was applied for 1 h (Alexa 488, goat anti rabbit, 1∶1000, Invitrogen, Karlsruhe, Germany).
Trypsin solution was applied for 5 min.
3% hydrogen peroxide solution was applied for 10 min to block endogenous peroxidase activity.
