After 20 days, the mice were euthanized, and their lungs were resected and photographs were taken (Nikon Coolpix 4500) before fixation in Bouin's solution for further analysis.
If plaques were formed, a single plaque from a successful infection plate was picked to propagate as a working stock solution for further analysis.
The 3D NMDS solution had substantially lower stress (0.24 instead of 0.35) and higher r (0.57 instead of 0.45) than the 2D solution, and showed clear groupings of sites and vegetation types, so we chose the 3D solution for further analysis.
The clusters were ranked by their size and scores, and the two most prominent solutions were chosen for further analysis.
The above colloidal solution was filtered and this solution was used for further analysis.
The final volume was made up to 25 mL, and the solution was used for further analysis.
Afterwards, the solid was centrifuged and 5 cm of clear solution were taken for further analysis.
The total lipids recovered were transferred to vials with 4 mL chloroform (stock solution), and stored at −18°C for further analysis.
The AgNp and Au Ag nanocomposite solutions were kept at room temperature for further analysis and usage.
BALL-SNP is able to support the user in selecting candidate nsSNPs for further analysis and finding possible solutions in both scenarios.
