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Polysaccharide extractions by 1) acidic sample solution with agitation, 2) flowing acidic sample solution, 3) static magnetic field combined with flowing acidic sample solution, and 4) rotary magnetic field combined with flowing acidic sample solution were conducted at pH level of 2 4, temperature of 30 50 °C, and treatment time of 0 180 min.
Then 24.5 mL aqueous solution of PVA (containing 0.5 g PVA) was poured into the KPSI solution with agitation and heated to 35°C.
Briefly, two 15 minute incubations at 40 to 44°C in an acetone/alcohol solution with agitation were followed by a 15 minute incubation at 64 to 66°C in a vacuum in a mineral oil paraffin reagent and finally another 15 minute incubation at 64 to 66°C in a vacuum in a paraffin reagent.
One gram of microspheres was suspended in 10 ml of EtOH NaOH solution with agitation on a plate rocker for 2 min, 3.5 min or 5 min, then poured onto a 50-μm sieve and rinsed with DW before transferring to a 50-ml centrifuge tube and centrifuged at 1400 rpm for 1 min.
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Silanization of the cleaned sensor chips was performed in the following way: toluene solution immersion with agitation for 30 s, followed by immersion of toluene with 4% trichloro methyl)silane solution for 1 h.
Then, PVP (0.33 g) was added into the solution with vigorous agitation for 30 min.
Next, 2.5 mL of a TEOS/ethanol solution (v/v = 1/4) were injected into the homogeneous solution, with intense agitation applied.
The radioactively labeled complex cDNA probes were hybridized overnight to Atlas™ mouse plastic 5 K arrays (Clontech, Palo Alto, CA) using ExpressHyb™ hybridization solution with continuous agitation at 68 °C.
Both soybean and lima bean seeds were surface sterilized in a 10% (v/v) bleach solution with slow agitation for 20 min, rinsed 4-7 times with sterile water and germinated between moistened sterile paper towels contained in GA7 culture vessels.
Two 6 mm tumor punches were minced in 5 ml of Dispase® (Becton Dickinson, San Jose, CA) solution and incubated with agitation for 1 h in a 37°C water bath.
The capsules were stored in the same solution at 4° with agitation for at least 1 hr or until they were used, then they were filtered and washed with distilled water to remove excess of calcium chloride.
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