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The silicon particles in the solution were subjected to subsequent ultrasonic dispersion of the colloidal solution for 30 min; as a result, the sizes of silicon particles in solution got distributed in the range from a few to 100 nm.
Six replicates each containing ten fish (kept in glass aquarium (size 20 × 10 × 12 in) containing 30 L of the test solution) were subjected to each concentration of lead nitrate (700, 750, 800, 850, 900, 950, and 1,000 mg/L) for the test.
Different specimens, made from a mixture of fly ash and rice husk bark ash in fine and coarse form together with alkali activator made of water glass and NaOH solution, were subjected to compressive strength tests at 7 and 28 days of curing.
100 µl of the solution were subjected to an enzymatic total bile acid measurement [36].
Cell lysates solubilized in Laemmli solution were subjected to SDS-PAGE on 8 or 10% resolving gels and immunoblotting as described [39].
Equal counts (or equal volumes) of the resulting solution were subjected to 10% silver nitrate-impregnated thin-layer chromatography (TLC) using silica gel 1D plates (5735 silica gel 60F254; Merck).
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The resultant salt solution is subjected to sieving or filtration.
The precipitate was discarded, and the clear solution was subjected to rotary evaporation to remove toluene.
The solution was subjected to reflux for 2 h in a boiling water bath.
The mixture of fruit extract and HAuCl4 solution was subjected to UV vis spectroscopy analysis.
The reaction solution was subjected to UV-visible (UV-vis) absorption measurements.
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