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Probes in hybridization solution were sealed under a cover slip on the sections, denatured for 3 min at 80 °C on a hot plate, followed by hybridization for >2 days at 37 °C in a moist chamber.
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Then the solution was sealed in autoclave and maintained at 180°C for 12 h.
Briefly, a volume of 1 mL NP solution was sealed in the dialysis bag (molecular weight cutoff 14,000 Da).
Then, the cylinder with the solution was sealed and was put in an oven in which the temperature was raised gradually to 170 °C.
We cannot rule out the possibility that a pH change occurred in the solution after it was sealed in the capillary tube, but evolution of hydrogen also was not observed after the solution was sealed in the tube.
After that, moderate urea was added, and the mixed solution was sealed in a beaker, and then it was heated at 80 °C for 3 h under vigorous mechanical stirring.
Following this, 5 mL of N2H4·H2O was added to the above system, and the solution was sealed in a 100-mL Teflon-lined stainless-steel autoclave for hydrothermal reaction at 150 °C for 6 h.
This solution was sealed and incubated for 60 minutes at 37°C.
Each solution was sealed in a quartz cuvette with a screw cap equipped with a rubber septum.
The solution was sealed between a second quartz plate and sandwiched between an aluminum housing with hand-tightened screws.
The solution was sealed and stirred 50 min at room temperature followed by stirring 24 h at 4 °C.
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