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After that, 2.5 mL of 4 N H2SO4 solution were added in each Erlenmeyer glass flask.
The reaction mixture was poured on ice-cold water and drops of ammonia solution were added.
After sterilisation, 5 mL of trace element solution and 1 mL of vitamin solution were added.
Flavonoids To the test solution, a few drops of sodium hydroxide solution were added.
Different concentrations of ferulic acid dissolved in DMSO stock solution were added.
Subsequently a certain amount of citric acid solution and phosphoric solution were added.
After culturing for 48 h, MTT solution were added to each well.
Afterward, 1.75 mL 0.5% hydrazine solution and 2 mL 2.5% ammonia solution were added.
On day 5, 30 μM of AlCl3 hexahydrate solution were added to half the containers.
Both trace element solution and vitamin solution were added using a sterile syringe filter.
Few drops of ammonia solution were added until pH 6 8.
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