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After coupling, 0.1% normal mouse serum was used for 2 hours before usage to capture the unbound GAMAP, after which the antibody solution was used on the slides.
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This solution was used for application on to the TLC plate.
The solid residue was filtered and 5 μl of the solution was used for HPLC analysis on a RP C18 column.
Twenty µL of a 50 mM cysteine/1 M NaCl solution was used to saturate unoccupied sites on the chip.
The resulting solution was used for stretching DNA fibres on silanized slides (Montpellier DNA Combing Facilities) at a constant speed of 18 mm/min.
The ligation solution was used to transform JM109 cells on an LB plate containing 50 µg/ml ampicillin, X-gal and IPTG.
The resulting cDNA was diluted 1 5 and 5μl of the resulting solution was used for Real Time PCR analysis on an Applied Biosystems 7300 machine using SYBR GreenER (Invitrogen).
All solutions were used on the day of preparation and all determinations were performed in triplicate.
All solutions were used on the day of preparation.
In Europe, mostly artificial colloid solutions are used on the basis of gelatin and hydroxyethyl starches (HES).
The most suitable dissociation solution was used later on during immunoaffinity extraction of OTA from wheat and cereal to dissociate OTA from the immunomagnetic microbeads.
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