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High-purity aluminium immersed in a neutral sodium chloride solution was submitted to galvanostatic pulses of different duration in a current density range between 10−5 and 10−1 A cm−2, as well as to a defined anodic current following immediately each cathodic pulse.
The initial molecular replacement solution was submitted to simulated annealing refinement.
Then, the carrageenan solution was submitted to two different treatments to obtain both low and medium molecular weight fractions.
After a 50-fold dilution with 1 N NaOH, the solution was submitted to spectrophotometric measurement at 280 nm.
After this period, the solution was submitted to centrifugation for 10 minutes (20,000 × g at 4°C).
This solution was submitted to the ARP/wARP server for automated refinement before iterative refinement was carried out in phenix.refine of the PHENIX software suite, REFMAC5 of CCP4, and Coot.
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As a control experiment, one of the oxidized cytochrome c solutions was submitted to a chemically reducing agent and conventional spectrophotometric measurements confirmed that 100% of the dissolved species could be chemically reduced.
These solutions are submitted to crossover and mutation operators.
The results of fresh mass of the plant, pH and electrical conductivity of nutrient solutions were submitted to 2-way ANOVA, considering winter and summer as the first factor and treatments as the second one.
The digest solution (4 μl) was submitted to a Prominence nano-LC System (Shimadzu) coupled to an AccuSpot LC spotting system (Shimadzu).
In order to measure the possible loss of the estrogenic activity due to the extraction and purification procedure, a solution containing 13 POPs (see below) was submitted to the extraction/purification procedure and the extracts were then analysed by reporter gene assay.
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