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The protein solution was split into seven batches of 10 mg each and contained 0.01 % sodium azide.
Factor V4A was first activated with α-thrombin, and the solution was split into three separate samples.
SDS was added to a final concentration of 0.3% and the solution was split into twelve 2 ml tubes (Eppendorf DNA LoBind, Eppendorf, Germany) and incubated for 20 min at 65°C followed by 30 min at 37°C under agitation.
Each template solution was split into two equal parts with half serving as an original template for the measurement of the damaged/relaxed mtDNA fraction and the other half heat-treated (95°C for 6 min on a PCR machine) to quantify total amount of mtDNA [ 31].
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The solution is split into a classical periodic solution and a complementary solution to account for stress-free boundary conditions.
These solutions were split into three aliquots: (1) 0.5 ml for preliminary Zn concentration measurements, (2) 1 ml for anion exchange chromatography to purify Zn for isotopic analysis, and (3) 1 ml for archive.
The final solution (~300 μL) was split into 2 aliquots per sample and frozen at −20 °C until analysis by 2D-LC-MS/MS.
The reaction was split.
Public opinion was split.
The numerical solution procedures are split into three steps: advection, diffusion and pressure propagation, and a Lagrange Euler method is used to track the free surface.
On C 2 c the solution could be split in the positive part x P ( t ) and negative part x N ( t ).
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