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For the autolysate fraction (including cell-wall and cell-membrane attached and intracellular biomolecules), approximately 4 g of washed wet mycelia were suspensed in 30 mL acetate buffer (pH = 4.7), completed with 100 µg/ml ampicillin and 50 µg/ml kanamycin, for 8 days at 24 °C and then the aqueous solution was separated by centrifugation (4 °C, 15000 RCF, 20 min).
The supernatant solution was separated carefully.
Then solution was separated from the undegraded agarose by centrifugation.
The phenol solution was separated out from the adsorbent filtering with 0.45 μm Whatman Millipore filter.
Then the dye solution was separated by centrifuge to determine the rest dye concentrations.
Then, Cu2+ in the solution was separated by displacement of iron power.
Similar(18)
Ultrafiltration (UF) is one of the pressure-driven separation processes by which some components of a solution are separated from the solvent by a semipermeable membrane.
When gold dissolution is complete, the gold-bearing solution is separated from the solids.
After leaching, the pregnant solution is separated from the insoluble residue and sent to precipitation.
Magnetic particles in solution were separated by means of bio-ferrography.
The internal reference electrode and reference solution are separated from the analyte solution by a membrane that is chosen to respond to the analyte.
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CEO of Professional Science Editing for Scientists @ prosciediting.com