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Exact(12)
Then the solution was sealed in autoclave and maintained at 180°C for 12 h.
Briefly, a volume of 1 mL NP solution was sealed in the dialysis bag (molecular weight cutoff 14,000 Da).
Then, the cylinder with the solution was sealed and was put in an oven in which the temperature was raised gradually to 170 °C.
Following this, 5 mL of N2H4·H2O was added to the above system, and the solution was sealed in a 100-mL Teflon-lined stainless-steel autoclave for hydrothermal reaction at 150 °C for 6 h.
After that, moderate urea was added, and the mixed solution was sealed in a beaker, and then it was heated at 80 °C for 3 h under vigorous mechanical stirring.
We cannot rule out the possibility that a pH change occurred in the solution after it was sealed in the capillary tube, but evolution of hydrogen also was not observed after the solution was sealed in the tube.
Similar(48)
Probes in hybridization solution were sealed under a cover slip on the sections, denatured for 3 min at 80 °C on a hot plate, followed by hybridization for >2 days at 37 °C in a moist chamber.
All solutions were sealed and stored at −20 °C until use.
Afterwards, series of the as-made solutions were sealed in a Teflon-lined stainless-steel autoclave.
Fmoc was removed from appropriate DESPS and SPS+ samples (B01 B04, B06, C01 C04, C06) by adding deprotection solution, the plate was sealed and incubated with shaking as before.
Fmoc was removed from appropriate DESPS and SPS+ samples (B08, C08) by adding deprotection solution, the plate was sealed and incubated with shaking as before.
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