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The mixture solution was refluxed for 24 h.
The resultant solution was refluxed at 100 °C.
Then, the solution was refluxed for 3 h under N2.
The reaction solution was refluxed for 23 h.
This solution was refluxed at room temperature for 24 h.
The resulting solution was refluxed with stirring for 10 h until the reaction was completed.
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The resulting solution was reflux for 10 h to obtain anatase sol.
The mixed solution is refluxing at 120°C for 4 h.
Oxidative degradation was carried out by taken 3 mL stock solutions of glycyrrhetic acid mixed with 3 mL of H2O2 (35% v/v) and the resultant solutions were refluxed for two hours at 80°C in the dark and 2 μL (1000 ng spot-1) of resultant solutions were applied on TLC sheet in triplicate and densitogram were developed.
For alkaline hydrolysis, 3 mL of methanolic stock solution of glycyrrhetic acid (1 mg mL-1) were mixed with 3 mL of each 0.1N, 1N and 5N NaOH separately and the resultant mixture solutions were refluxed for two hours at 80°C in the dark. 2 μL (1000 ng spot-1) of 0.1N, 1N and 5N NaOH treated solutions of glycyrrhetic acid were applied on TLC sheet in triplicate and densitogram were developed.
Solutions were refluxed for 2 h at 65 °C.
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