Sentence examples for solution was overlaid from inspiring English sources

After washing with PBS, 20% glycerol solution was overlaid and cells counted under a microscope.

Fifty microlitres of protein solution was overlaid on the sucrose cushion and incubated at 30°C before centrifugation at 300  g for 3 min.

A total of 20 µL of either solution was overlaid on each well and allowed to air dry prior to larval infestation.

For imaging experiments, a 1 mM solution of Chloroxynil in IMB was made and 100 μl of this solution was overlaid onto to seedlings mounted in 100 μl of agarose, yielding a final concentration of 500 μM.

A solution of 120 mg ml−1 polyHEMA (Sigma) in 100% ethanol was made and diluted 1 10 in 95% ethanol; 0.95 ml mm−2 of this solution was overlaid onto 35-mm wells and left to dry in a heated dryer system for 12 h.

For the more friendly isolation of products and for better removal of conducting salt a biphasic preparative electrolysis was developed, where the electrolyzed acetonitrile solution is overlaid by hexane.

Beclin 1 ECD (10 μM) and 1.2 mg/ml LUVs were mixed with stock sucrose (68% w/v) to generate 1 ml 50% (w/v) sucrose liposome-protein solution, which was overlaid with 1 ml 40% (w/v) sucrose and 0.25 ml buffer containing 50 mM Na2HPO4/NaH2PO4 (pH 7.4).

The samples were pelleted down at 40,000 rpm (172,000×g) in 50 Ti rotor and resuspended in 1 ml of solution A. This resuspended pellet was overlaid on a three-layer sucrose gradient made up of 3 mls each of 2, 1.5 and 1 M sucrose and centrifuged at 44,000 rpm (200,000×g) in 50 Ti for 2 hrs.

The preparation was overlaid with 2 µL of matrix solution [saturated solution of α-HCCA (alpha-cyano-4-hydroxycinnamic acid) in 50% AN and 2.5% TFA].

Each sample was overlaid with 1 µL of matrix solution (saturated solution of HCCA (alpha-cyano-4-hydroxy cinnamic acid) in organic solvent (50% acetonitrile and 2.5% trifluoroacetic acid) and air-dried.

The spot sample was overlaid with 0.5 μL of matrix solution (saturated solution of alpha-cyano-4-hydroxy-cinnamic acid) in organic solvent (50% acetonitrile and 2.5% trifluoroacetic acid) and air-dried.