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Therefore, in stage II of the study, the addition of NaHCO3 8.4% to the BNP7787 solution was omitted.
Joulie's solution was omitted in the morning of operation and was readministered as soon as bowl function returned after the operation.
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In this method, the function evaluation of a solution is omitted when the fitness of its nearest point in the search population is worse than that of the compared point.
For the Ca2+-free extracellular solution, CaCl2 was omitted from the above solution or 3 mM EGTA and 1 mM CaCl2 was used to clamp free [Ca2+] at ∼100 nM, as indicated.
For Ca2+-free solution (0 Ca2+), Ca2+ was omitted.
Calcium-free buffer was a modified bath solution in which CaCl2 was omitted and 0.5 mM EGTA was included.
The effect of 2,4-dinitrophenol (DNP; 10 mM) on K+ current in H9C2 cells was measured using perforated patch-clamp electrophysiology with essentially the same pipette solution as above just ATP was omitted and amphotericin B (Sigma, 240 μg/ml) added.
The constituents of Ca2+-free solutions were the same as the bathing solution described above except that CaCl2 was omitted and 0.1 m M EGTA was added to eliminate possible Ca2+-contaminations of the other constituents.
Sorbitol was omitted from the base solution to form the hypotonic solution.
The same fixation solution was used except that glutaraldehyde was omitted.
For Fe-deficiency, Fe+3-EDTA was omitted from nutrient solution and samples were collected after 7 days.
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