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The following day, the acidic solution was neutralized with 0.5 mL of 2 mol⋅L−1 NaOH and 5 μL of the neutralized solution from each group was placed in 96-well plate.
The resultant solution was neutralized with ammonia and the metal ions were then titrated against EDTA.
The mixture was stirred for another 30 min for complete reaction then the solution was neutralized.
The solution was neutralized with ammonia to pH = 7 and was then cooled to room temperature.
The solution was neutralized with 1 N HCl, and the formed precipitate was filtered (Additional file 1).
After the alkali ultrasonication, the solution was neutralized and centrifuged at 5,000 rpm for 5 min.
Afterwards, the solution was neutralized on ice by fast addition of 1 mL 1 M K2HPO4 and 0.9 mL 5 M KOH (Buchholz et al. [2001]).
After completion of the stress the solution was neutralized by using 5 M NaOH and completed up to the mark with mobile phase.
After completion of the stress the solution was neutralized by using 5 M HCl and completed up to the mark with mobile phase.
The derivatization reaction was conducted at an ambient temperature for 10 min in the dark, and then the solution was neutralized with 1.0 M KOH and the pH of the solution was controlled to 9.5 with 0.2 g of NaHCO3/K2CO3 (2:1, w/w).
The light brown solution was neutralized by Na2CO3 and then dialyzed against Millipore pure water (Billerica, MA, USA) for 3 days to remove the salt of sodium through a dialysis membrane with an MW cutoff value of 1,000, affording purified carbon nanoparticles.
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