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Briefly, 8 L of alginate/cell solution was mixed with a density modifier4,6,7.
The resulting solution was mixed gently and incubated for 5 15 min at room temperature.
While the solution was mixed gently, the sericin hydrogels were formed within approximately 60 seconds.
Nine mL of each inactivating solution was mixed with 1 mL of 2% chlorhexidine solution.
Then solution was mixed.
The solution was mixed for 2 h and centrifuged.
Then this solution was mixed with above solution.
The solution was mixed overnight at 25 °C.
The concentrated solution was mixed with alcohol (2.8 vol) to obtain the crude polysaccharide.
The solution was mixed and the absorption was measured at 510 nm.
Then, permethrin solution was mixed with Kempas (Koompassia malaccensis) wood powder and Rubberwood (Hevea brasiliensis) powder.
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