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The resulting solution was layered by the oil and water phase.
Bumetanide solution was layered on sugar pellets followed by coating with various coating formulations according to a central composite statistical design using a Glatt GPCG 1 Wurster Fluid Bed.
3 ml of the solution was layered on a 35 mm NGM plate and dried at room temperature.
Forty mls of blood/salt solution was layered on top of 10 mls Ficoll-Paque Plus (Amersham Biosciences) and centrifuged at 400×g for 40 minutes at 20°C.
The pellets were each dissolved in 1 ml wash buffer and 2 ml of the chloroplast solution was layered carefully on top of sucrose gradients, prepared by using 30% and 52% sucrose solution, respectively.
Briefly, blood diluted 1 1 with saline solution was layered over 5 mL Lymphoprep (Nycomed Pharma AS, Oslo, Norway).
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Then, aqueous sample solution containing THP was layered over the organic phase and the lid was closed.
After centrifugation at 4°C (106 × g, 80 s), the supernatant was layered onto solution II containing 460 mM mannitol, 140 mM sucrose, 1 mM EGTA, 2 mM PMSF and 10 mM HEPES, pH 7.4, and centrifuged (800 × g, 3 min).
Working with experienced doctors to fully understand the ins and outs of a traditional in-person visit will help create a baseline for how a telemedicine solution can be layered into the process.
This kind of problem, in 2011, is unacceptable, so Krishna and company devised a solution that is layered over existing accounting applications and integrates directly with the bank's payment systems without making changes to either.
6 ml of the solution was carefully layered on a sucrose cushion consisting of 5 ml of 30% sucrose solution in a 15 ml centrifuge tube and centrifuged (2,500×g, 5 min, swinging bucket rotor).
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