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The solution was incubated for 30 min, followed by 90 min of incubation.
100 μL of primary anti-elongin B mAb diluted (1 500) in blocking solution was incubated overnight at 4 °C.
Solution was incubated for 10 min in darkness.
The solution was incubated for 30 min at 95 °C in a heating block.
The reaction solution was incubated at room temperature for at least 16 h with gentle shaking.
The solution was incubated at room temperature and was observed by a naked eye.
For the RCA process, the mixed solution was incubated at 30 °C for 20 h.
Then the solution was incubated at 25°C and observed at different time.
The mixed solution was incubated with gentle shaking at room temperature for 6 h.
After this time, the solution was incubated at room temperature for 3 h.
The final solution was incubated at room temperature for 2 h.
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