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Oriented iodine-doped nylon 6 specimen which was prepared in an I2KI aqueous solution was immersed into a KI aqueous solution.
As a good dope solution was immersed in a harsh bath, e.g., water, precipitation occurred initiated by liquid liquid phase separation.
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For the sample preparation, the glass substrates pre-cleaned with piranha solution were immersed in 10% (v/v) solution of (3-aminopropyl) triethoxysaline (APTES, Sigma Aldrich) in anhydrous ethanol for 20 minutes, rinsed for five times in ethanol with sonication and dried at 120°C for 2 hours.
The cell containing solutions was immersed in a water bath, controlling the temperature variation at ± 0.1°C.
Then the frozen polysaccharide solutions were immersed in their respective non-solvents to form porous scaffolds.
The two flasks containing the identical aqueous HAuCl4 solutions were immersed in the ultrasonic bath for 10 min to stir and heat up the solutions.
The strength of the solution in which the bone was immersed for the first two weeks was 1 1000; thereafter, it was kept in a solution of 1 5000 which was changed every two weeks until the bone was used.
After an equilibration period of 24 h, saturated calomel electrode (SCE) was immersed into solution containing steel rebar.
Then the Au/AuNPs/PNA modified electrode was immersed into solution containing ZnSe-QDs (pH 7.4) in presence of 0.1 M EDC for 5 h to form Au/AuNPs/PNA/ZnSe-QD electrode.
In brief, 2 g of CEG (or CGP) and 1.5 g of NaNO3 were added into 150 mL of 98%H2SO44 solution in a flask which was immersed in an ice bath.
After each of detection, the electrode was immersed into aptamer solution for regeneration.
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