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The coarse gum solution was homogenized using a high pressure homogenizer (APV, Crawley, UK) for two cycles at different pressure levels (30 and 25 MPa).
The solution was homogenized and heated in a 60°C water bath to improve solubility.
The solution was homogenized by shaking at 0°C and deoxygenated by bubbling nitrogen through it for 20 min.
Afterward, the solution was homogenized 3 min using a homo mixer at 4,000 4,500 rpm.
After the addition of 5 mL of distilled water, the solution was homogenized and filled to 10 mL with distilled water.
The solution was homogenized, after which the metallic carbonate (Ca2+, Sr2+, and Ba2+) was dissolved and a stoichiometric amount added to the molybdenum citrate solution.
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The collected cells in 0.5% KH2PO4 solution were homogenized by sonication.
To measure glycosaminoglycan (GAG) and DNA content in the NP and AF, samples in Ambion® KDalert™ lysis buffer solution were homogenized in a tube rotator O/N at 4 °C, whereas samples in Complete Lysis-M EDTA-free buffer were homogenized in a TissueLyser II (Qiagen) for 2 × 30 s at 20 Hz.
Lungs free of blood by in situ perfusion (1.5 ml/min warm Hank's solution) were homogenized in 150 mM NaCl, 50 mM Tris, pH 8.0 and protease inhibitors; total lysates were prepared by adding NP-40, to a final concentration of 1.0 %, for 2 h, at 4 °C.
Thirty milliliters of each CSGH solutions was homogenized with 10 mL of soybean oil for 1 min at room temperature (25 ± 1°C) using Moulinex R62 homogenizer.
Finally, the composite solutions were homogenized with a magnetic stirrer for 1 h at room temperature.
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