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An equimolar quantity of NaOD (1 M, aq) was added, and the solution was gently stirred for 30 min until a clear solution was formed.
The solution was gently stirred and incubated at 80 °C for 30 minutes.
The solution was gently stirred for 5 min while the droplets were cured.
No stirring was necessary after the solution was gently hand shaken (approximately 1 min) for homogenization.
The solution was gently evaporated on a steam bath until a residue was left.
The solution was gently stirred and titrated at 37 °C with ammonia solution to pH 9.
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This solution is gently brushed between and over every petal.
The poured tannin solution is gently stirred for about 10 20 seconds, after which the water inflow is again resumed.
Briefly, 2 μg plasmid DNA and 6 μl Fugene solution were gently mixed in 80 μl serum-free RPMI 1640 medium and incubated for 1 hour.
0.5 ml of each individual gradient solution were gently layered into a 4.5 ml centrifuge tube (Beckman, 344062) from the heaviest to the lightest solution, as previously described [ 40].
Freshly grown bacterial cells, suspended in the 10mM MgCl2 solution, were gently infiltrated (about 50 μl/spot) from lower side of the leaves into the intracellular space by using a syringe.
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CEO of Professional Science Editing for Scientists @ prosciediting.com