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The ethanolic extract was concentrated under reduced pressure to small volume and the solution was fractionated with a HPD-826 macroporous adsorptive resin column eluting with H2O and 90% EtOH.
The protein solution was fractionated between 40% and 60% saturated ammonium sulfate.
The IgG solution was fractionated from the rabbit antisera by precipitation with 40% saturated ammonium sulphate and then affinity-purified on peptide affinity columns.
The supernatant solution was fractionated in a DEAE-Sephacel column (2.5 × 20.0 cm) previously equilibrated with 50 mmol/L ammonium bicarbonate (AMBIC), pH = 7.8.
Similar(56)
The purified phytase solution (PPS) was fractionated by one-dimensional SDS-PAGE (10%).
The CsCl gradient was fractionated into 22 fractions through bottom puncture of the centrifugation tubes.
A solution of the precipitate in a minimal volume of acetone was fractionated by flash chromatography (CHCl3/acetone, 8 1 → 4 1 gradient).
Aqueous HCl solution (0.1 M, 5 mL) was passed through the generator, and the eluate was fractionated (5 × 1 mL).
The resulting solution was cleared by centrifugation at 22,000 × g for 40 min at 4°C, and the supernatant was fractionated with solid ammonium sulphate between 35 and 65% saturation.
Protein samples were prepared in 1× Laemmli buffer solution and heated at 95ºC for 10 minutes, and 20 μg/lane (determined by Bio-Rad Protein Assay) of protein was fractionated on sodium dodecyl sulfate polyacrylamide gels.
Non-axenic soil was fractionated by buoyant density centrifugation.
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