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Directly after removing the endoscope from the patient, a sterile saline solution was flushed down the biopsy channel to provide a 'before clean' estimate of bioburden.
As described in the experimental section, firstly the pure acetate buffer solution was flushed over the crystal surface (baseline), stage I, followed by a contact-period with the laccase-maltodextrin suspension, stage II.
The flask was put into a water-bath at 60 °C and the solution was flushed with nitrogen gas for 30 min, and then the initiator solutions were added into the flask.
A syringe containing approximately 1 MBq/cm3 FDG solution was attached to a catheter as used for the shunt [7], and FDG solution was flushed through the catheter which was guided through the coincidence counter.
During hospitalization, every lumen of the CVC (3 ml) was locked for 15 minutes per day, following which the solution was flushed through with 10 ml 0.9% NaCl.
From 2005 through 2008, each lumen of the CVC of adult hematology patients was locked for 15 minutes per day with either 70%-ethanol or placebo, where after the lock solution was flushed through.
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Even with a reversible seal, fluid flow was perfectly controlled; at a flow rate of 300 µl h−1, solutions were flushed completely from the reaction channel after only 5 s.
After injection of the solution, the column was flushed with H2O, 0.05% TFA (v/v) for 3 min. Peptides were eluted with an increasing gradient of acetonitril (AcN), 0.05% TFA (v/v) (0 50% in 70 min and 50 100% in 17 min).
After 10 min of incubation, the enzyme solution, containing the ECs, was flushed from the aorta by perfusion with DMEM (37°C) from one syringe through the vessel into the other syringe.
Prior to putting them in salt solution, a dry stream of N2 was flushed for at least 15 min to control the reaction kinetics, which are strongly related with the oxidation speed of the iron species.
First, after measurement of the pressure that induced sap flow in untreated roots, the pressure was released slowly before opening the chamber, the cut stem was removed from the gasket and the pot was flushed with HgCl2 solution.
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