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The chloroform fraction was recovered and the solution was filtered through a silica gel column.
The solution was filtered through a Millipore Millex-LH 0.45 μm (Millipore) to remove particulates.
This cell solution was filtered and incubated with PI (1 μg/ml) at room temperature for 10 minutes.
Before use, the SiNPs solution was filtered through 0.22 μm cutoff membrane filter, then its concentration was detected.
After 60 min the solution was filtered.
Now the whole solution was filtered.
The solution was filtered under vacuum.
Then, the solution was filtered and dried.
The solution was filtered and boiled.
The centrifuged solution was filtered through 0.45-μm filter.
The resulting solution was filtered to obtain the samples.
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