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First, the alginate solution was emulsified in a chloroform solution containing PLGA (water-oil) with the model drug (i.e. calcein) contained within the alginate solution.
After mutual saturation, the solution was emulsified for 20 min under ultrasonification (ULH700S, Ulssohitech, Cheongwon-gun, South Korea) at 450 W. The mixture was kept overnight at room temperature to remove the volatile organic solvent.
The resulting solution was emulsified in 40 ml of PVA 2.5% to 5% (w/v) aqueous phase solution by homogenization (homogenizer, IKA ULTRA-TURRAX T-25, IKA Labortechnik, Staufen, Germany) at 8,000 rpm for 10 min. A large volume (200 ml) of deionized water was added dropwise into the previous solution to promote the diffusion of ethyl acetate into the aqueous phase.
rAdglob in Ringer's solution was emulsified in Freund's complete adjuvant (FCA; Sigma) by Luer-locked double syringe mixing.
4-OHT solution was emulsified in sunflower seed oil (Sigma-Aldrich) by vortexing, which was followed by mixing on a rotator for 4∼6 hours.
Tamoxifen solution was emulsified in corn oil (Sigma-Aldrich C8267) at 10 mg/ml by vortexing.
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Equal volumes of collagen solution were emulsified with FCA and IFA [ 12].
Aqueous monomer solutions were emulsified in kerosene with a blend of two surfactants (Span80 and OP10).
According to a 5-factorial 3-level Box Behnken type experimental design aqueous solution of BSA was emulsified in an immiscible organic phase composed of dichloromethane and various quantities of dissolved PLGA to get water-in-oil (w1/o) emulsion.
The immobilized library was then resuspended in the amplification solution, and the mixture was emulsified, followed by PCR amplification.
For the preparation of Rh B-loaded NPs, a 200 μL aliquot of an aqueous Rh B solution of specified concentration was emulsified in the organic phase for 5 min using a high speed homogenizer (Polytron PT4000, Littau, Switzerland) to produce a w/o emulsion.
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