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Following cation exchange, the reaction solution was dissolved in chloroform, and then acetone was added to precipitate the QDs.
Then, an ascorbic acid polyol solution was dissolved under the same conditions.
Twenty microliters of 3%% ethanol iodine solution was dissolved in 10 ml of buffer.
White residue, appear in the solution, was dissolved by adding NaOH solution and finally, the clear solution was obtained.
The HSA stock solution was dissolved and diluted to 1.0 × 10−5 mol L−1 with the same buffer, then was stored in the dark at 4 °C before fluorescence and UV vis absorption essay.
For total FAME analysis, 0.2 mL of each oil extract (stock solution) was dissolved in 1 ml toluene and then methylated with 1% sulfuric acid in methanol (2 ml), using a 15 mL screw-cap Pyrex culture tube at 80°Cfor 2 h.
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Samples in solution were dissolved at a known concentration, and dispensed into a 1 cm path-length, quartz cuvette and tested for absorbance between 280 and 360 nm.
10.0 g of the ascorbic solution were dissolved in 100 mL DI water.
Lyophilized protein samples extracted with TS and NaCl solution were dissolved in deionized water (6.25 mg protein/ml).
Before measuring gadolinium concentration, samples of colloidal solution were dissolved in concentrated nitric acid for 24 hours.
After that, aliquots of 20 µL of the stock solution were dissolved in 500 µL of distilled water.
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