Sentence examples for solution was discarded from inspiring English sources

After 40 minutes, the blocking solution was discarded and the membrane was incubated with a binding buffer, Solution F. After washing, the chemiluminescent substrate was incubated with the membrane for two to five minutes at room temperature.

After 4 h incubation, unreduced MTT solution was discarded.

The supernatant solution was discarded and the precipitate preserved.

The solution was discarded and the pelleted particles were again suspended in 20 mL hexane.

The supernatant solution was discarded, and the precipitate was washed thrice with ethanol.

The transparent upper solution was discarded and replaced with solutions of the three dyes of interest (15 mL, 25 μM).

The MTT solution was discarded, and 800 μL DMSO was added to each well in order to lyse the cells.

The solution was discarded every 3 days and the process repeated till the end of the study.

The black products were then precipitated by centrifugation at 8000 rpm for 10 min, and the supernatant solution was discarded.

To avoid best so far solution is discarded in scout bee stage.

At that point, 170 µL of solution were discarded and 100 µL of DMSO (Sigma-Aldrich, USA) was added to all wells.