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A semi-continuous set-up was designed in which the donor solution was continuously fed into the autoclave reactor containing the glycerol aqueous phase.
The solution was continuously stirred and heated to 75 °C to ensure complete metal complexation.
The obtained solution was continuously stirred at 70°C for 1 h.
After mixing with ASW, the mixed aqueous solution was continuously stirred.
Under N2 ambience, the mixture solution was continuously stirred at 150°C for 2 h.
Ringer's solution was continuously infused as maintenance fluid (10 ml/ kg h)).
The solution was continuously stirred at fixed temperature for an assured time to attain the equilibrium.
The solution was continuously pumped in a closed cycle by a flow of 1.5 L/min.
The solution was continuously agitated using a magnetic stirrer to produce a black precipitate.
The solution was continuously stirred and purged with nitrogen gas for 30 min to remove oxygen.
Ringer's solution was continuously infused as maintenance fluid (10 mL·kg-1·h-1).
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